Margaret of Brandenburg (18 April 1453 – 27 April 1509, Hof) was a princess of Brandenburg by birth and abbess of the Poor Clares monastery at Hof from 1476 to 1490.
After her mother's death, however, Margaret was betrothed to Count Palatine Kaspar of Zweibrücken.
But then Margaret fell dangerously ill and decided to dissolve her betrothal and become a nun after all.
Her younger half-sister Amalie was then betrothed to Kasper and married him in 1478.
In 1476, Margaret succeeded Catherine of Kotzau as abbess of the Poor Clares monastery at Hof.