She lost her father when she was the age of seven, and was brought up at Aix-en-Provence by her grandfather René of Anjou.

When she was relieved of the duties imposed upon her by her position, she decided to renounce the world and retired to Mortagne, to a monastery of religious women who followed the rule of Saint Elizabeth.

Later, having brought with her to Argentan some of these nuns, she founded there another monastery which she placed, with the authorization of the pope, under the rule of Saint Clare, modified by the Minor Observants.

On 2 November 1521, after having lived an austere life for a year, she died in her modest cell, at the age of fifty-eight.

After an invitation made by the bishop of Séez, Jacques Camus de Pontcarré, Louis XIII asked Pope Urban VIII to order a canonical inquiry into the virtues and the miracles of the Duchess.