Margaret of Münsterberg (25 August 1473, Breslau – 28 June 1530, Dessau) was a German regent: Duchess of Anhalt by marriage to Prince Ernest I, she ruled the principality as a regent for her underage sons from 1516.
Margaret was the fourth child of the Duke Henry the Elder of Münsterberg (1448–1498) and his wife Ursula of Brandenburg (1450–1508).
Ernest died in 1516 and Margaret took up the regency of the principality for her underage sons John IV and George III.
In 1525, Margaret launched the League of Dessau, an alliance of Catholic princes opposed to the Reformation.
Margarethe of Münsterberg managed to regain some manors located east of Wörlitz, that had been pledged to neighbouring Electorate of Saxony.