Gładkowska portrayed Daisy, Princess of Pless, her breakthrough performance in Magnat (1987), which was included in the list of 100 Best Polish Films of all time.

Her first professional stage appearance was as Elwira in a production of Husband and wife (Polish: Mąż i żona) by Aleksander Fredro (1983).

[2] Her most notable stage appearances are as Mother in The Laments (2001), as Mary Tyron in Long Day's Journey into Night (2006), as Susie Cameron in The Spirit Level (2011) opposite Andrzej Szczytko, as Alice in Sexy Laundry (2018) opposite Wojciech Wysocki, and as Anne Hathaway in Shakespeare's Will (2019).

Additionally, Gładkowska has appeared in many Television Theatre (Polish: Teatr Telewizji) plays including Irydion (1982, dir.

She has three children, including Adam Wróblewski, known for his role in the Janusz Majewski's autobiographical film Mała matura 1947 (2010).