Two themes stand out in her cinematic work: motherhood, which she explored in Post-Partum,[5] Passages,[6][7] Femelles,[8] and Your Mother is a Thief!

;[9] and artistic creation, with McLaren's Negatives,[10][11][12] The Sapporo Project, Jutra,[13] Snowflakes, Oscar, and The Lauzon Theory.

[17] She also served as artistic director and animator on Patricio Henríquez's feature documentary Uyghurs: Prisoners of the Absurd.

[21] Marie-Josée Saint-Pierre is also an author on women and animated cinema: she has published books and scientific articles on the subject.

[22][23][24] As part of her academic research, she led the creation of two animation series, developed in collaboration with students under her supervision.