Studholme was born in Eccleshill, Bradford, West Yorkshire, England, the only child of Joseph Ludholme Lupton, an auctioneer, and his wife Emma Greaves.

[1] She was raised in Baildon by her mother, her paternal grandparents and in Shipley by her father's two half-sisters, one being Mrs. Frank Rhodes,[2] and educated at Salt Grammar School on the Saltaire mill estate.

She understudied Letty Lind as Daisy Vane in An Artist's Model, eventually taking over the role and recreating it in the New York production in 1895.

[1] By 1913, Studholme was appearing in music hall comedy sketches, including one entitled "Her Ladyship", at the Wood Green Empire in London, and touring in variety shows.

She had great success and popularity in the British provinces, and returned triumphantly to Bradford to perform at the opening of the town's Alhambra music-hall in 1914.

[1] Amid the demands of a busy theatre schedule, Studholme found time in 1907 to study Jujitsu with Yukio Tani.

[1] In 1904, Studholme brought and won a lawsuit against Edward Foley, a London dentist, who altered her photographic image for use in an advertisement without permission.

[20] She converted to Christian Science and lived out her retirement in Hampstead and Laleham where, in 1909, the young Architect Edward Maufe designed a weekend house for her.

[1] Studholme died at her home in London in March 1930 from a short but virulent attack of rheumatic fever, at the age of 57, and was buried in the St Marylebone Cemetery in East Finchley, survived by her second husband and children.

[1] On 10 March 2012 The Music Hall Guild of Great Britain and America erected a commemorative blue plaque at Studholme's last home in Hampstead, and restored her memorial at St. Marylebone Cemetery in East Finchley.