In the following Greek-Italian War (October 28, 1940 to April 7, 1941), he was posted to the 22nd Pursuit Squadron, based on the airfield of Thessaloniki.

During the dogfights, three of the bombers were shot down, while the rest reached their targets, and then started to return to their base in Albania.

[3] In September 1948, during a routine training flight in an Airspeed Oxford, he died crashing in the south Aegean Sea.

[3] At the outbreak of the Greek-Italian War on 28 October 1940, the Greek Air Force was severely outnumbered, counting only 79 aircraft against the 380 fighters and bombers available to the Italian Regia Aeronautica.

[1] Mitralexis' feat boosted Greek morale, being depicted in several artists' impressions, in newspapers and magazines, as well as on a postage stamp.