The Mark Twain Boyhood Home & Museum is located on 206-208 Hill Street, Hannibal, Missouri, on the west bank of the Mississippi River in the United States.

Other highlights of the museum properties include the Boyhood Home garden area, bordered by a high stone wall built by the WPA.

On May 15, 2012, to commemorate the 100th anniversary of the museum, a spokesperson announced the establishment of the "Mark Twain Lifetime Achievement Award" with Hal Holbrook named as the first recipient.

It features Jimmy Buffett as Huckleberry Finn, Garrison Keillor as narrator, Clint Eastwood as Mark Twain, and Angela Lovell as Susy Clemens.

Singers include Emmylou Harris, Doyle Lawson & Quicksilver, Rhonda Vincent, Bradley Walker, Carl Jackson, The Church Sisters, Sheryl Crow, Brad Paisley, Marty Raybon, Val Storey, Vince Gill, Joe Diffie, and Ricky Skaggs.

The town of Hannibal celebrates National Tom Sawyer Days over the 4th of July each year complete with whitewashing and frog jumping contests.