Born in Zipaquirá, Martha Lucía Zamora graduated from the Universidad Externado, and worked as a professor at Sergio Arboleda and Saint Thomas Aquinas universities before beginning a long career in the Judiciary of Colombia.

A year later, Judge Alejandro Martínez Caballero took her as an assistant magistrate to the Constitutional Court, and there she was the promoter of several guardianship decisions, such as the sentence that eliminated the chepitos (debt collectors), and the first action that ordered a school to readmit a pregnant girl.

Likewise, she was the manager of a key decision that determined the minimum conditions of imprisonment for the mentally ill.[1] She then joined the Prosecutor's Office during Gustavo de Greiff's administration, becoming the first female attorney delegated to the Supreme Court.

On 31 May 2012, prosecutor Zamora took on the investigation of the young University of Los Andes students Laura Moreno and Jessy Quintero, involved in the Colmenares case, after Antonio Luis González was removed by order of Attorney General Montealegre.

She worked as a legal researcher of the International Commission Against Impunity in Guatemala (CICIG), one of the Justice Support Institutions of the United Nations, and served as its chief from October 2016 to July 2017.