She is the executive editor and anchor of The Story with Martha MacCallum, broadcast from Manhattan Monday through Friday at 3PM ET, and co-anchor of Fox News Election coverage.

After graduating from Ramapo High School in Franklin Lakes, New Jersey,[2][3] MacCallum earned her bachelor's degree in political science from St. Lawrence University in the North Country of New York State, and subsequently studied at the Circle in the Square Theatre School on Broadway, founding the Miranda Theater Company in New York.

The program averaged 3.5 million viewers in the first month, an increase of 79 percent in the time slot compared to the year before.

[9] In 2022, MacCallum began hosting The Secret History of World War II with untold stories about heroes from WWII, with three seasons and eleven episodes.

The interview aired on The Story With Martha MacCallum on September 24, 2018 at 7 PM ET  and was watched by 3.6 million viewers, cable news' most-watched program of the night.

"[16] She likened the storming of the U.S. Capitol to a peaceful protest outside Josh Hawley's home a few days earlier; The New York Times said it was "bizarre" to link "swarms of Trump supporters breaking into the seat of democracy, an event that made worldwide news, to a small-scale incident at the home of a Republican official.

[18] In 2019, MacCallum and Bret Baier moderated numerous town halls with potential presidential candidates in advance of the 2020 election.

[25] Martha MacCallum moderated the first Republican debate of the 2024 election season with Bret Baier at the Fiserv Forum in Milwaukee, Wisconsin on August 24, 2023.

In attendance was Ron DeSantis, Nikki Haley, Tim Scott, Chris Christie, Vivek Ramaswamy, Mike Pence, Doug Burgum, and Asa Hutchinson.

[26] In January 2024, MacCallum co-moderated with Brett Baier three back-to-back town halls featuring Donald Trump, Ron DeSantis and Nikki Haley.

In 2024, MacCallum received the John R. "Tex" Cray Award for Excellence in Journalism from the Congressional Medal of Honor Society for her work covering the military.