Marthasville is a city in Warren County, Missouri, United States.

The Katy Trail, a 240-mile long bike path, passes through Marthasville.

Because of the many wineries from here east to Defiance, Marthasville is considered to mark one end of the "Missouri Weinstrasse".

[6] The Herman H. Fortmann Building, Marthasville Hardware Building, and Starke-Meinershagen-Boeke Rural Historic District are listed on the National Register of Historic Places.

[9] Due to the rich soils and steep slopes, the landscape is highly suitable for viticulture, growing grapes and maintaining vineyards.

There were 478 housing units at an average density of 562.4 per square mile (217.1/km2).

23.9% of all households were made up of individuals, and 9.3% had someone living alone who was 65 years of age or older.

22.4% of all households were made up of individuals, and 8.4% had someone living alone who was 65 years of age or older.