[5] Biron, along with Rob Ray and Dominik Hašek, was one of the three Sabres against whom, in three consecutive years, the NHL made a specific rule.

Biron became the Sabres' starting goaltender after Hašek's departure from Buffalo in 2001 and held the position until the 2004–05 NHL lockout.

Biron has always been a fan favorite in Buffalo and although he no longer plays there, he currently resides in the city and after his retirement has a position as director of goaltending at HarborCenter, which is owned and operated by the Sabres organization.

After losing the number one goaltending job to emerging youngster Ryan Miller, Biron requested a trade from the Sabres on June 26, 2006, in an effort to receive more playing time.

[7] On March 27, 2007, Biron signed a two-year, $7 million contract extension with the Flyers to be the starting goaltender in Philadelphia.

Biron soon signed a one-year, $1.4 million contract with the New York Islanders on July 22, 2009,[8] joining goaltenders Dwayne Roloson and Rick DiPietro.

On July 1, 2010, Biron signed a two-year, $1.75 million deal with the New York Rangers, where he prepared to back-up Swedish starter Henrik Lundqvist.

While he played in the lowest number of games (17) since his second NHL season in 1998–99, he posted career highs in save percentage (.923) and goals against average (2.13).

[10] On October 14, 2013, the Rangers placed Biron on waivers after a poor start to the season,[11] and the following day, he was assigned to play with the Hartford Wolf Pack of the American Hockey League (AHL).