He worked as a teacher from 1912 to the end of 1913 and subsequently at the Ethnographic Museum in Santiago de Chile with Max Uhle until 1922, becoming a head of department in 1918.
The objective was to explore the different groups of Tierra del Fuegan people, the Yamana and Selk'nam (also known as Ona), who had been displaced by immigrants and severely depleted by imported diseases for which they lacked natural resistance.
Together with Ferdinand Hestermann, he edited and helped arrange for publication in 1933 of a Yamana-English dictionary, based on an 1879 manuscript by Rev.
He ended his career in research, lecturing and teaching activities at the Mission St. Gabriel in Maria Enzersdorf, Vienna.
In Puerto Williams, Chile, the Martin Gusinde Anthropological Museum was erected in his honour, which records his work with the Tierra del Fuego people.