It briefly follows Pennsylvania Route 297 over Interstate 83 and through the village of Strinestown, and continues on rural roads to an escarpment above the Susquehanna River.

[7] The Mason-Dixon Trail continues to generally follow the Susquehanna, except for a trip inland at Muddy Creek and its substantial side gorge, with a brief walk along Pennsylvania Route 74 near the village of Castle Fin.

At Havre de Grace, the trail crosses the Susquehanna via US 40 and now heads to the east via various rural roads and occasional footpaths through Cecil County.

The trail crosses the border into Pennsylvania and follows footpaths in and around White Clay Creek Preserve and undeveloped areas in southern Chester County.

The trail passes back into Delaware for a walk along various roads in the Hockessin area, followed by a brief segment through Auburn Valley State Park.