Mastyugino (Russian: Мастю́гино) is a rural locality (a selo) and the administrative center of Mastyuginskoye Rural Settlement, Ostrogozhsky District, Voronezh Oblast, Russia.

[3] Mastyugino is located 47 km north of Ostrogozhsk (the district's administrative centre) by road.

Novo-Uspenka is the nearest rural locality.

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