After impressing in the 2018 Copa São Paulo de Futebol Júnior, he was promoted to the first team,[2] and made his senior debut on 17 January of that year, coming on as a second-half substitute in a 2–2 Campeonato Paulista Série A3 home draw against Barretos.
[3] Regularly used as a defensive midfielder as the club suffered relegation, Matheus Alexandre moved to Ponte Preta shortly after, returning to youth football.
[8] Regularly used in the 2021 Campeonato Paulista, Matheus Alexandre missed the quarterfinals match after not being allowed to play against his parent club.
[14] On 30 December 2022, Matheus Alexandre agreed to a three-year contract with Cuiabá also in the top tier, with Corinthians and Ponte Preta also retaining a part of the player's economic rights.
[15] He immediately became a first-choice at his new side, and scored his first professional goal on 1 April 2023, netting his team's fourth in a 5–0 Campeonato Mato-Grossense home routing of Luverdense.