Mathilde Marchesi (née Graumann; 24 March 1821 – 17 November 1913) was a German mezzo-soprano, a singing teacher, and a proponent of the bel canto vocal method.

She was generally an advocate of a naturalistic style of singing: she called for a fairly instinctive method of breathing and argued against the "smiling" mouth position that many teachers of her day preferred.

She was particularly concerned with vocal registration, calling it "the Alpha and Omega of the formation and development of the female voice, the touchstone of all singing methods, old and new."

She also repeatedly expressed disdain for the teachers of her day who offered methods that they asserted would fully develop the voice in only a year or two.

Her "analytical method" placed great importance on intellectually understanding both the technical and the aesthetic nature of everything sung, from grand arias to simple vocal exercises.

Her daughter, Blanche Marchesi (1863–1940), a contralto, also a noted singer and teacher, made her début at a young age.