Its storyline bears some resemblance to real-life instances of gender imbalance and economics resulting in fraternal polyandry and bride buying in some parts of India.

[1] It depicts a future in an Indian village populated exclusively by males due to female infanticide over the years.

[2] Matrubhoomi received widespread critical acclaim[3] and was shown at festivals through 2003, including the 2003 Venice Film Festival, where it was presented in the Critic's Week (Parallel Sections) and later awarded the FIPRESCI Award "For it's [sic] important theme on women's issues and female infanticide handled with sensitivity by a first-time director".

The now uncouth and aggressive young men of the village are desperate for wives and release their frustration through group screenings of imported pornographic films, cross-dressed dance performances, and even bestiality.

A sympathetic domestic servant boy, from lower caste community, helps her to escape but he is brutally murdered by the brothers, while Kalki gets recaptured by them and they chained her to a post in a cow shed.

The lower caste community of the village hold her responsible for the death of the servant boy and decides to avenge the murder through gang rape.

As the news spreads, every man in the area claims paternity of the unborn child, which causes violence to break out in the village.

Subsequently, while surfing the web, he read an article mentioning the fact that over the years, millions of girl children had fallen victims to gender discrimination in India.

[9][10] The cast included actors from Delhi theatre circuit, Sushant Singh, Aditya Shrivastav, Piyush Mishra and Deepak Bandhu.