In his second year of single-seater racing, Nannini moved up to the FIA Formula 3 Championship with Jenzer Motorsport.

Nannini joined ART Grand Prix for the second mid-season test at Barcelona, replacing Juan Manuel Correa who was out due to a foot injury.

Nannini was set to perform double duties in both F3 and Formula 2 in 2021, partnering Alessio Deledda at HWA Racelab.

[11] Williams Academy driver Jack Aitken replaced Nannini for the Monaco and Baku rounds.

On March 22, 2022, Team Stange Racing had announced that they would be returning to the ARCA Menards Series with their No.

[14] On July 18, 2023, it was announced Juncos Hollinger Racing and driver Matteo Nannini had ended their INDY NXT by Firestone competition relationship.