Medenine (Arabic: مدنين Mednīn; French: Médenine) is one of the 24 governorates (provinces) of Tunisia.

The governorate includes the country's largest island, Djerba, which is connected by a ferry boat and has over a third of the total population of the governate and its own airport.

The northern projection abuts Kebili Governorate and includes part of a long escarpment which extends from the south of Gabes Governorate (adjoining Medenine to the north) to beyond Tripoli in Libya and which is dotted with settlements.

Precipitation is low and reaches a maximum between October and January, the far north of the governorate and western hill range benefiting from this most.

Key settlements are those associated with ports, tourism, fishing, farming in more fertile parts within the north-east and oases.