Mesilat Zion (Hebrew: מְסִלַּת צִיּוֹן, מסילת ציון, lit.

Located near Beit Shemesh with an area of 1,000 dunams, it falls under the jurisdiction of Mateh Yehuda Regional Council.

[1] The moshav was established on the land of the depopulated Palestinian village of Bayt Mahsir in 1950.

It symbolizes the breaking of the siege over Jerusalem during the 1948 Arab–Israeli War, it is based on verses from the Book of Isaiah, chapter 62, "For Zion's sake will I not hold my peace, and for Jerusalem's sake I will not rest....Go through, go through the gates; prepare ye the way of the people; cast up, cast up the highway; gather out the stones; lift up a standard for the people....Say ye to the daughter of Zion, Behold, thy salvation comes.

"[3] The Jerusalem Culinary Institute (JCI) founded in 2001 by chef Yochanan Lambiase is located in Mesilat Zion.