Her collegiate career was played with the Clarkson Golden Knights of ECAC Hockey, with whom she twice won the NCAA Championship.

Pejzlová joined a talented Clarkson team that had won the National Championship three years previous and made the NCAA Tournament four straight seasons.

Pejzlová recorded 11 goals and 21 assists as the team's highest-scoring rookie en route to Clarkson's second National Championship.

2016–17 Awards Pejzlová played the majority of the 2017–18 season centering Loren Gabel and Élizabeth Giguère on Clarkson's top line as well as centering the pair on the top power play unit.

2017–2018 Awards Pejzlová represented the Czech Republic at the IIHF Women's World Championships in 2016, 2017, 2019, 2021, 2022, 2023, and 2024, and at the Division I Group A tournament in 2015.