Microbiology Spectrum is a peer-reviewed scientific journal published by the American Society for Microbiology.

Topics the journal covers include: archaea, food microbiology, bacterial genetics, cell biology, physiology, clinical microbiology, environmental microbiology, ecology, eukaryotic microbes, genomics, computational and synthetic microbiology, immunology, pathogenesis, and virology.

[1] The journal was established in October 2013.

In Spring 2021, it became an online-only open access publication.

[2] The journal is abstracted and indexed in: According to the Journal Citation Reports, the journal has a 2023 impact factor of 3.7.