Most of the time, the kill is performed with the powerful mandibles literally biting through the head of the prey causing either death or immobilization.

There might also be a situation where the trophallaxis benefits the adults but also hinders the larva in form of nutritional castration where the lack of food results in underdeveloped reproductive organs thus leading to the creation of a worker class.

Species usually form communal nests that hang from a strong support such as a tree branch, vine, or overhanging rock formation via a petiole which is produced from a root tip rather than silk.

The nests are typically built with substrate surface material such as bark, lichens, and sand grains interlaced with silky secretion produced by the female wasps.

Most of the infant holding cells are the near the bottom and the adults reside in the upper hollow section of the next close to the entrance.

In Microstigmus thysanoptera, the adults form a compact mass of prey provisions and silk fibers which, upon hatching, the larva consumes.

[10] The fecal matter produced by the larva that have molted is removed from the nest along with other trash by the adults performing housekeeping duties.

One is the conical supranal process which is potentially an adaptation for obtaining the prey provisions stuck on the walls of a nursery cell.

[13] Other aspects of Microstigmus biology are shared with other genera in the family Pemphredonidae such as antennal papillae, lack of galeae, and tridentate mandibles which indicate close relation to Spilomena and distant from Ammoplanus.

Along with the inspections, adults also perform the maintenance routes where the wasps remove particles and waste and check the integrity of the nest.

The key to this is that the genetic structure spawns nonrandom genotypes which coupled with differential reproduction makes kin selection possible.

[14] Inter-nest relatedness was high resulting in few independent haploid genomes which makes possible the development of a social hierarchy.

Relatedness between individuals drastically decreases over a few generations and as such, mating in Microstigmus colonies tend to be low to the point where it is not uncommon to see a nest with only one female with fully functioning ovaries.

Reproduction within Microstigmus nests from various subspecies where some has a queen-worker divide and the queen's sole purpose is to reproduce, while others have specific females rear the offspring.