As with similar games of that era, Midnight Mutants features a large in-game environment with many locations, a background musical soundtrack, battles against boss enemies and an animated introduction.

On Halloween night in 1992 young Jimmy Harkman's grandfather (known as "Grampa") has been imprisoned inside of a pumpkin by a resurrected villain named Dr.

With Doctor Evil on the loose, Jimmy finds the countryside has become inhabited by scary creatures such as zombies and werewolves that can injure him physically and also make his blood impure.

Midnight Mutants was developed by Radioactive Software, a team consisting of programmer and designer Peter Adams; writer Tammy Moore; artist Les Pardew; and composer Paul Webb.

"[7] In a retrospective review, Atari 7800 Forever gave the game a 4.5 out of 5, praising the humor, the approachable in-game menus and the excellent boss battles.

It summarized, "This isometric arcade adventure has a spooky Halloween-based plot, which is told by an impressive animated intro, and a huge map that will have you playing right into the midnight hours.