Millbrook is a locality in the state of Victoria, Australia, located 90 kilometres (56 mi) north west of Melbourne.

Millbrook adjoins or is near to other districts of the Moorabool Shire such as Ballan, Egerton, Gordon, Wallace and Bungaree and its early history is inseparable from theirs.

The first European colonists in the region were graziers known as the "Over-Straiters", who had originally come over Bass Strait from Van Diemen's Land (now Tasmania) in the 1830s.

This involved the acquisition of land from neighbouring landowners, a cause of some controversy, particularly in view of claims that the lessening of travel time would be minimal.

On the Eastern Fringe: Recollections and Stories on the Settlement and Farming Histories in the Bungaree, Wallace, Bolwarrah and Gordon Districts.