Maps and station numbering use navy blue and the route symbol MM to identify the line.

Operations are managed by Tokyu Railways, with no changing of staff at Yokohama Station; Tokyu staff (train drivers and conductors) continue to support the train over the Minatomirai portion of the line as well.

Station-side staffing is provided by Minatomirai employees with the exception of Yokohama Station, which is managed by Tokyu Railways.

Train management is controlled by Yokohama Minatomirai Railway Company at an operational command center.

Along with the link to the Fukutoshin Line being established, Tōkyū Tōyoko and Minatomirai express trains (limited express/commuter limited express/express) were expanded from eight-car to ten-car configuration (with the exception of a portion of regular express trains).

Regular trains stopping at each station on the line continue to operate in the eight-car configuration.

Limited Express (特急, Tokkyū) trains run during daylight hours during the week and at all times excepting early morning and late night on weekends and holidays.

Commuter Limited Express (通勤特急, Tsūkin Tokkyū) operate during the morning rush hour and in the evening on weekdays.