In general, the genus Mischocyttarus can be identified by the asymmetrical internal and external lobes of the thetarsal segments in adults.

Similarly, mature Mischocyttarus larvae are identified by appendix-like projections extending forward from the abdominal sternum.

[6] M. m. cubicola is similar to other species such as M. flavitarsis and M. angulatus as they all have a sharp secondary margin on their pronotum, the dorsal surface of the prothorax.

In the southernmost part of its range, Mischocyttarus mexicanus cubicola nests through the year, whereas in the more temperate regions, it hibernates during the winter season.

In addition, it has been hypothesized that as M. mexicanus cubicola individuals age, they accumulate a specific odor, which aligns with the observation that older female wasps are usually rejected from foreign nests.

[11] It has also been found that male individuals of this species have exocrine gland cells in their gastral sternites, which supports the idea the M. m. cubicola uses chemicals for communication such as nest-mate recognition and pheromone signaling.

In addition, when the number of potential habitats in a give area is experimentally decreased, the nests are more likely to be constructed by pleometrosis and the converse has also been found to exist as well.

These observations suggest that the M. mexicanus species reject foreign wasps based on the risk of nest usurpation.

[1] Consequently, due to the high variability in females, nest switching is common where if a queen is removed, a resident may quickly replace her over the course of a week.

[8] Mischocyytarus larvae can be distinguished from other wasps by having a one-, two-, or three-pointed lobe on the ventral surface of the first abdominal segment.

It has been hypothesized that these abdominal lobes play a role in feeding, with similar morphology to the trophthyllax of pseudomyrmecine ant larvae.

[2] The cannibalism of this species seems to show that when the queen is unaided by workers, she resorts to nourishing herself by feeding on her brood and their saliva rather than by foraging.

[9] Major sources of predation of Mischocyttarus mexicanus cubicola are birds such as wrens, blue jays, yellowthroats, cardinals, mockingbirds, and woodpeckers.

Ant species such as Campanotus floridanus, Crematogaster ashmeadi, and Pheidole floridana have been observed to raid live nests and eat the wasp colony's brood.