It travels approximately 168 miles (270 km), serving Humphreys, Leflore, Carroll, Grenada, Yalobusha, Lafayette, Marshall, and Benton counties while serving several points of interest, including Florewood River Plantation State Park, the University of Mississippi, and Wall Doxey State Park.

MS 7 now travels through the community of Swiftown and the town of Morgan City, where it passes just a few miles west of Mathews Brake National Wildlife Refuge.

MS 7 now comes to an intersection with US 82, which it becomes concurrent with and they head east as four-lane divided highway to enter the Greenwood city limits, immediately passing along the northern edge of Florewood River Plantation State Park.

US 49E joins the concurrency and they cross a floodway (which dumps water from the Tallahatchie River into the Yazoo) to enter a business district.

I-55/MS 7 head north as a four-lane freeway to cross the Yalobusha River and have an interchange with Papermill Road (Exit 208).

It then has an intersection with MS 328 before entering the Oxford city limits and passes through neighborhoods for a few miles to an interchange with US 278/MS 6, which provides access to the main campus of the University of Mississippi (Ole Miss).

The highway now passes by Wall Doxey State Park before becoming concurrent with MS 4 and entering the Holly Springs city limits.

MS 4/MS 7 enter town along S Craft Street and widen to a four-lane highway for a short distance to have an interchange with I-22/US 78 (Exit 30) and pass through a business district.

The highway narrows to two-lanes and passes through neighborhoods for several blocks to make a right turn onto W Van Dorn Avenue and enter downtown.