The route travels eastward in a straight line for over two miles (3.2 km) before turning northeast.

From there, Route 16 heads northward, crossing over North Fabius River, and enters Monticello.

The road passes through the center of the village and meets Route A as it turns east.

The road begins to shift northward, and three miles (4.8 km) later, Route 16 crosses over Sugar Creek.

At Wyanconda River, the route begins to shift back southward, and enters the city limits of Canton.

The road enters downtown Canton, and ends at a junction with Route B and US 61 Business.