[2][3] Knight is the first Black female superhero in Marvel comics; DC introduced a character named Nubia a year earlier.

As private investigators, Misty Knight and Colleen Wing frequently worked with the Heroes for Hire duo Luke Cage and Iron Fist.

Simone Missick portrayed the character in the Netflix television series Luke Cage (2016–2018), The Defenders (2017), and the second season of Iron Fist (2018), set in the Marvel Cinematic Universe (MCU).

[7][8] Misty Knight was a rising star with the NYPD when she was seriously injured preventing a bomb attack that forced the amputation of her right arm.

Misty aided Iron Fist and Power Man in rescuing captives of John Bushmaster and procured a videotape clearing Cage of crimes.

During the 2006 "Civil War" storyline, Misty Knight and Colleen Wing were contacted by Iron Man, Reed Richards, and Spider-Man to re-form Heroes for Hire and track down superhumans who refused to register.

Initially hesitant, the pair eventually agree creating a team including Shang-Chi, Humbug, Orka, Black Cat, Paladin, and a new Tarantula.

[29][30] After the Civil War between the superheroes ended, Iron Fist was shocked to find that Misty had sided with the Initiative as he had joined the New Avengers.

After being captured by Warbound, Colleen Wing and Tarantula were offered to No-Name the Brood Queen by their possessed teammate Humbug.

[41] During the 2010 "Shadowland" storyline, Misty, Colleen Wing, Iron Fist, Luke Cage, and Shang-Chi confront Daredevil in an attempt to stop him without violence.

[42] Misty teams up with Paladin, Silver Sable and the Shroud to discover who is framing the Hand for the murder of a number of New York's mobsters.

[46] Misty Knight appears during the 2015–17 All-New, All-Different Marvel promotion as a supporting character to Sam Wilson, the new Captain America, who is uneasy now that his friend Steve Rogers has regained his original moniker.

Though the two men opt to share the name, many civilians in the Marvel Universe feel that Sam Wilson is undeserving of the title.

[47] She then helps clear the names of female heroes and villains, who fell victim to a scandal over embarrassing sex videos posted on the internet.

[49] Using an Attilan Security Force Skycharger that was "borrowed" from the Inhumans, Daredevil, Misty Knight, Nur, and Cypher investigate the sightings of Wolverine in Manhattan, Phoenix, and Chicago.

Misty Knight and Nur arrive at McCarthy Medical Institute in Manhattan where Jane Foster was enrolled and learned that an unnamed man delivered flowers.

When they arrive, Daredevil, Misty Knight, and Nur find the males dead and the female missing as they head into the forest to investigate.

Upon returning to Chicago, Daredevil, Misty Knight, and Nur visit the security guard she questioned again only to find him dead and a bomb nearby as it goes off.

His analysis revealed that the security guard worked for a group called Soteira which was listed as an asset management company.

As Nur asks what he plans to do with the drive after getting him, Misty Knight, and Cypher back to New York, Daredevil says that he is giving it to Kitty Pryde while informing her on who else is looking for Wolverine and what they are walking into.

[53] During the "Blood Hunt" storyline, Misty Knight and Lizard's Isotope Genome Accelerator duplicate enlisted Spider-Man in locating Colleen Wing and Morbius the Living Vampire.

[54] Misty Knight is a highly skilled combatant who, in addition to her police-combat training, is proficient in martial arts and possesses near-perfect aim with firearms.

She is a superb detective, having graduated at the top of her class at the police academy and earned a degree in criminology from the John Jay College of Criminal Justice.

[55] Her bionic arm is superhumanly strong, and she can punch a target with incredible force, or crush objects as tough as steel in her vise-like grip.

Her new arm was built by Stark Industries and is an alloy of Antarctic vibranium and diamond; at close range it can liquefy all known metals, including adamantium.

Showcasing an energy shield to ward off incoming assaults with, a force field which she can expand over a marginal radius to help protect multiple people if need be.

[59] Deirdre Kaye of Scary Mommy called Misty Knight a "role model" and a "truly heroic" female character.

[61] John Wilson of WhatCulture ranked Misty Knight 4th in their "10 Best Comic Book Detectives (That Aren't Batman)" list.

In DC Comics, she was preceded by Teen Titans member Mal Duncan who debuted in 1970, Green Lantern wielder John Stewart (1971), and Mister Miracle protégé Shilo Norman (1973).

[volume & issue needed] In Ultimate Mystery, Misty Knight later became a scientist and appears as a member of Roxxon Brain Trust.