The museum mainly displays traditional costumes, Buddhist artefacts, sculptures, palm-leaf manuscript, handicrafts, bronzeware, silverware, coins and figurines of the ethnic Mon people who founded one the earliest civilisations in Mainland Southeast Asia, and were responsible for the spread of Theravada Buddhism in Mainland Southeast Asia.

Essentially, the museum houses ceramic art of Mon kingdoms including Martaban jars and Mon musical instruments that can only be seen in the world famous museums.

Moreover, it exhibits a manuscript copy of Myazedi Stone Inscription (AD 1113) in Mon language and a replica of Ceremonial helmet of Queen regnant Shin Sawbu (the real one is at the V&A Museum, London).

The museum also displays artefacts of the Pyu people, Taungoo culture and Konbaung culture including the 19th century divans used by Konbaung dynasty's Queen Seindon and Princess Myat Phaya Galay, who resided in Mawlamyine in the late 19th century or the early 20th century.

[2][3] Museum's opening hour is 09:30 AM – 04:30 PM (Tuesday to Sunday) except public holidays.