The current rail-over-road Mooroolbark station structure was opened on 19 November 2021, as part of railway redevelopment works, including the Level Crossing Removal Project.

Mooroolbark station opened on 10 October 1887[4] and, like the suburb itself, is named after an Indigenous word meaning 'red earth'.

[5] The name came from a cattle run that was leased to John Gardiner, with the land stretching from Brushy Creek to Olinda.

[5] In 1949, flashing light signals were provided at the former Manchester Road level crossing, which was located at the up end of the station.

The project was scheduled to be completed by 2022, however, on 19 November 2021, the rebuilt station opened to passengers.