The bridge carries the Amtrak Northeast Corridor trains and SEPTA Trenton Line as well as non-revenue trains for NJ Transit's Northeast Corridor Line that have terminated at the Trenton Transit Center bound for the Morrisville Yard.

A series of Pennsylvania Railroad (PRR) predecessors operated trains across the nearby Lower Trenton Bridge from 1834 until 1903, when PRR completed a grade separation project through Trenton, including this stone arch bridge on a new alignment.

[1] In 1953, the Morrisville approach to the bridge was blocked when eight cars of an eighty-three-car freight train operated by the Pennsylvania Railroad derailed at 11 p.m. on January 13.

No one was injured in the incident; however, four of the derailed cars were described in news reports as "smashed across the four tracks," along with a steel pole that "was knocked across the tracks, tearing down the lines feeding current to express and local trains."

As a result, the railroad's main line to New York was inoperable for five hours, forcing the cancellation or delay of more than twenty passenger trains.