The Moyaone is bordered to the north by Piscataway Creek, to the east by MD Route 210, and to the west is Marshall Hall Road.

These included the Moyaone Association, a non-profit civic organization, and the Alice Ferguson Foundation which runs Hard Bargain Farm.

The Accokeek Foundation runs the National Colonial Farm, which is adjacent to the Moyaone Reserve and within Piscataway Park.

In 1960, the Washington Suburban Sanitary Commission (WSSC) wanted to build a water treatment plant in Mockley Point, which was Accokeek shoreline.

Its purpose was to "preserve lands which provide the principal overview from the Mount Vernon Estate and Fort Washington" in order to designate 133 acres (54 ha) around Mockley Point, which was to be the site of water treatment plant, as a national landmark.

The resolution also authorized the National Park Service to receive donations and scenic easements from adjacent communities.

In 2019, the Moyaone Association applied for designation of the neighborhood in the National Register of Historic Places in part after a battle with Dominion Energy over the siting of a natural gas plant.

[1] The Moyaone Reserve's approach to conservation was described by Maryland Republican Senator John Marshall Butler as "an almost unique or pilot project in cooperative individual activity for the development and use of the countryside without destroying its natural attraction.

For a period of time, the Accokeek Foundation held the conservation easements before they could be transferred to the federal government in the early 1970s.