It fell into obscurity until it was satirized in an episode of Mystery Science Theater 3000 that first aired on November 30, 1991, after which it was celebrated as one of the best shorts in the history of the show.

In captions for the productions photos included in the three-page essay, the title character is consistently referred to as "he", an indication that Conn intended Mr. B to be of the male gender.

Buzz shows an interest in music like the more popular kids at school, but is so shy that he makes excuses to not attend a dance, even when a girl directly invites him.

In visiting the music dealership, Buzz's parents are reassured by the salesman that buying a trumpet is "simply making a small investment in your son's lifetime personality."

When Buzz mentions that he didn't care what make his new horn would be, he is reproached by Mr. B Natural, and is treated to a detailed description of the C. G. Conn factory and laboratories.

Betty Luster was in Chicago in the last months of 1956 as a featured dancer-singer in the revue "Hey Day" at the Palmer House's Empire Room,[13] which would suggest that her segments in Mr. B Natural were filmed during this period.

In 1991, HBO sought content for its new network, Comedy Central, and employed "ephemeral film" expert Rick Prelinger to locate amusing vintage footage.

Following the short, Joel moderates a mock-serious debate between Crow T. Robot and Tom Servo as to whether Mr. B Natural is a man or a woman.

Examples include: Mr. B was recreated by Bridget Jones in the live broadcast "Turkey Day" host segments of episode #701T Night of the Blood Beast, on November 23, 1995.

In 2013, RiffTrax, a comedy website that features three of the stars of Mystery Science Theater 3000 (Michael J. Nelson, Bill Corbett, and Kevin Murphy), wrote and performed an all-new commentary track for Mr. B Natural.