Mueller–Hinton agar

Mueller Hinton agar is a type of growth medium used in microbiology to culture bacterial isolates and test their susceptibility to antibiotics.

The composition of Mueller Hinton agar can vary depending on the manufacturer and the intended use, but the medium is generally nutrient-rich and free of inhibitors that could interfere with bacterial growth.

For example,  MH agar may be used in the laboratory for the rapid presumptive identification of C. albicans, as an alternative method for germ tube test (Mattie.

It typically contains: Five percent sheep's blood and nicotinamide adenine dinucleotide may also be added when susceptibility testing is done on Streptococcus and Campylobacter species.

Mueller–Hinton agar was co-developed by a microbiologist John Howard Mueller and a veterinary scientist Jane Hinton at Harvard University as a culture for gonococcus and meningococcus.

Colonies of Burkholderia pseudomallei on Mueller–Hinton agar after 72 hours incubation.