He is best known for his portrayal of Shaktimaan, the superhero character in the self-created television series Shaktimaan (1997–2005) and Bhishma in BR Chopra's Mahabharat (1988–1990), and also for his appearances in Pyaar Ka Dard Hai Meetha Meetha Pyaara Pyaara (2012–2013).

Khanna was born into a Punjabi Hindu family with roots in Multan and Dipalpur, cities located in today's Punjab province of Pakistan, from where his family migrated to Mumbai (then Bombay) due to the 1947 partition.

[4] Following his higher education at Mumbai's St. Xavier's College, earning a Bachelor of Science (B.Sc.)

degree and a Master of Laws qualification, he later completed an acting course from the Film and Television Institute of India (FTII) in Pune.

[7] He is currently running The Mukesh Khanna Show on his own YouTube channel, Bheeshm International.