The 16339 / 16340 Mumbai CSMT–Nagercoil Express runs between Chhatrapati Shivaji Maharaj Terminus (CSMT) railway station at Mumbai, Maharashtra, and Nagercoil Junction in Nagercoil, Tamil Nadu.

The main cities on the way are Tirunelveli, Virudhunagar, Madurai, Salem, Katpadi, Pakala, Dharmavaram Anantapur, Guntakal, Adoni, Solapur, Pune, Lonavala, and Thane.

Still, after COVID, it was rerouted via Katpadi, Pakala there by completely skipping Bangalore, due to which Bangalore lost Mumbai Connection and Southern Tamil Nadu Connection simultaneously.

This train departs Nagercoil at early morning and travels through Tirunelveli, Madurai, Dindigul, Karur, Salem, Katpadi, Pakala, Dharmavaram, Guntakal, Wadi, Solapur, Pune, and reaches its destination Chhatrapati Shivaji Terminus (Mumbai CSMT) on next late evening.

From Katpadi it is hauled by Kalyan-based WDP-4 / WDM-3D twins till its destination Chhatrapati Shivaji Terminus (Mumbai CSMT).