Four large paintings depicting the cloth industry by Isaac van Swanenburg hang in the same spots on the walls as designed.

Similarly, a grand over-the-mantel piece by Carel de Moor shows the inspectors in a massive wooden frame decorated with their family shields, flanked by a series of three historical allegories of the city of Leiden by Abraham Lambertsz van den Tempel.

The museum hosts a collection of altarpieces and religious artifacts from before the Protestant Reformation that were formally ceded to the state in 1572.

The collection also includes A Pedlar Selling Spectacles (Allegory of Sight), one of a series of five, The Senses, by Rembrandt.

[7] On its reopening in June 2019, and until February 2020, the museum had on display a newly identified painting by Rembrandt, Suffer little children to come unto me, showing Jesus preaching.