The Museum was founded in 1991 by members of the Czech Romani intelligentsia, led by Jana Horváthová, in the period of freedom which followed the Velvet Revolution in Czechoslovakia.

In December 2000, it moved to its present building on Bratislavská Street in Brno, which is the centre of the local Romani community.

In cooperation with the Moravian Gallery, the Museum of Romani Culture has prepared the exhibition The Universe is Black in the Governor's Palace.

[2] In September 2020, the newly emerging specialized workplace of the museum was introduced - the Roma and Sinti Center in Prague.

The family house was designed by architects Arnošt (Ernst) Mühlstein and Victor Fürpro in 1937 for Leo František Perutz, a textile entrepreneur.