Directed by Masakazu Obara and written by Hiroyuki Yoshino, it consists of four episodes, which were released across three-month intervals and is the sequel to the original My-Otome anime series.

At Otakon 2013, Funimation Entertainment had announced that they have rescued My-Otome Zwei, along with a handful of other former BEI titles.

Arika is now a full-fledged Otome (though still under the tutelage of Miss Maria as she has not yet received enough credits to graduate) and Nagi is incarcerated in a prison somewhere in Aries.

A mission to destroy a meteor threatening to collide with Earl sets into motion a chain of events which result in a mysterious shadowy figure attacking Garderobe and several Otome as well as a new, more powerful version of Slave appearing across the planet.

The series follows Arika's search for Mashiro as well as Garderobe's attempts to uncover the truth behind the shadowy figure.