[2] In 1874, Nadezhda returned to Russia and married Nikolai Ziber, at that time an assistant professor of the Department of Political Economy and Statistics at St. Vladimir University in Kyiv.
In 1875, after Nikolai Ziber retired, they moved to Switzerland, where Nadezhda continued to study medicine at the University of Bern.
[3][1] After her graduation, she worked for 4 years in the laboratory of Nencki, and, in 1884, Nadezhda was chosen to be an assistant in physiological chemistry at the University of Bern.
Their research was based on a series of results on the decomposition of hemin into products carried out by Nencki's group over the years.
She also came to St. Petersburg at July 21- August 2, 1890, apparently, for negotiations with Duke Alexander of Oldenburg about the possibility of future work in the Institute of Experimental Medicine.
As a result, a modern research center was created, that surpassed similar European scientific departments and laboratories of that time.
Together with a team of like-minded people, Nencki and Ziber-Shumova launched scientific research in physiological chemistry, designed to maintain links between medicine and biology.
[1] In the 1890s, during the period of numerous epidemics, efforts were made in Russia to organize a health care system and sanitary control.
In April 1895, she was awarded a bonus of 1000 rubles for her work on the development of diphtheria vaccine at the Imperial Institute of Experimental Medicine.
Later, in 1895 and 1898, Nadezhda Ziber-Shumova took a part in expeditions to the Caucasus, the purpose of which was fighting against rinderpest - an infectious viral disease of cattle.
[1] Her works, including those written in collaboration with Nencki, discuss the chemical composition of various pigments of animal tissues, blood pigments and their derivatives, oxidases and other enzymes, the biology of fermentation and decay, the chemical composition of bacteria, toxins and antitoxins , the pathogenicity of microorganisms, etc.
[1][5] In addition to her scientific activity, Ziber-Shumova did a lot for the further development of science with the help of funds inherited from her uncle.
[1] Due to her academic achievements and scientific works, she is the most notable person of that time in the history of Russian Science.
One of the confirmations of the importance of her contribution to science is the lifetime publication of her biography in the Brockhaus and Efron Encyclopedic Dictionary.