Initially used as an airliner, the militarized version was used by the Imperial Japanese Army in a variety of roles, ranging from medical evacuation to transport and training aircraft.

Due to its rugged durability, long-range flight ability and large cargo capacity, the Fokker Super Universal was extensively used in bush flying and was well regarded for its versatility.

Nakajima also sub-licensed additional production to its subsidiary Manshūkoku Hikōki Seizo KK in Manchukuo, which also produced an unknown number of units.

[1] This was followed by an order for twenty trainers to be used for training pilots, gunners, bombardiers, and wireless operators.

The aircraft built in Japan were used for both civil and military roles with some remaining in operation until after World War II.