N. vitripennis females have a straight stigmal vein (a short branch from the stigma of the forewing), in comparison to the varying curvature in its three sister species.

Male N. vitripennis wasps have a spindle-shaped scape (the lower half of the antenna), meaning it is wider in the middle than at either of the joint ends.

Prior research has pointed to the rectal papillae (inside the rectal vesicle) for the purpose of water and electrolyte resorption, since the adult male wasps rarely feed; however, localization techniques, pheromone biosynthesis data and observations of wasp behaviour (tapping abdomen on the ground, leaving traces of pheromone) all point to these organs being used in sexual communication.

[3] Cephalic pheromones are also present in N. vitripennis, coming from the mouth of the males during courtship, which females contact with their antennae while signaling their receptivity to mating.

[7] Egg-laying in N. vitripennis occurs in the same fashion in all known species of Nasonia, where a mated female drills into and deposits her eggs under the puparum of fly larvae.

[6][10] It has been suggested that Wolbachia have assisted with genetic divergence through buildup of mutations and selecting for compensatory mechanisms, causing the emergence of new strains to occur more quickly.