[2][3] In 1908, the school moved to Chicago, because its founder desired a scientifically more rigorous academic culture.

[4] Another reason that Howard relocated to the Chicago area was that he received an agreement allowing his students to have access to anatomical study of cadavers at the nearby Cook County Hospital.

After being chartered and incorporated by the State of Illinois, the college's first home was on Congress Street across from Presbyterian Hospital.

In 1927, the college established its first clinic, the "Chicago General Health Service", which remained in operation until 2008.

[9] The National University of Health Sciences publishes three scientific journals for the chiropractic profession:[10]