The National Museum of San Matteo in Pisa (Museo Nazionale di San Matteo) displays works from historic ecclesiastical buildings in the city and Province of Pisa.

It has a rich collection of antique paintings, including works by Berlinghiero Volterrano, Giunta Pisano, Simone Martini, Lippo Memmi, Francesco Traini, Masaccio, Beato Angelico, Benozzo di Lese, and Ghirlandaio.

The nucleus of the collection was initially started by a 1796 endowment by the Canon of the Pisa Cathedral, Sebastiano Zucchetti.

Further works were added after the Napoleonic closure of many religious establishments, and through the Academy of Fine Arts.

In 1893, a local Jewish citizen, Igino Benvenuto Supino, helped found the Pisan Museo Civico.