The northernmost segment begins at Ellsworth and goes north to the South Dakota border.

The southern segment of Nebraska Highway 27 begins at the Kansas border.

It goes north 0.78 miles (1.26 km) through farm fields to Haigler, where it intersects U.S. Highway 34 and ends.

[1][3] The middle segment of Nebraska Highway 27 begins at Interstate 80 at Exit 95 near Chappell.

[1][5] The segment of Nebraska Highway 27 between Ellsworth and Gordon runs through cattle range of the famous Spade Ranch and passes notable Nebraska writer Mari Sandoz home and gravesite.