Negoro-ji (根来寺) is a Buddhist temple located in the city of Iwade, Wakayama Prefecture in the Kansai region of Japan.

He also attempted to assert the authority of Mount Kōya over the temple's metropolitan headquarters at Tō-ji in Kyoto.

These reforms led to animosity form various reactionary political factions within the Shingon hierarchy, and facing calls for his expulsion, he resigned his posts in 1135 and retired to the chapel of Mitsugon-in (密厳院).

[3] However, the animosity against him continued, and after armed followers of the other factions burned down his residence in 1140, he fled further south into the mountains of Kii Province to an estate which he had received in 1132 from ex-Emperor Toba called Ichijō-zan Daidenpon Negoro-ji.

The temple enjoyed a good relationship with Oda Nobunaga, having assisted him during the Ishiyama Hongan-ji War from 1570 to 1580 and with Tokugawa Ieyasu during the Battle of Komaki and Nagakute in 1584; however, it earned the enmity of Toyotomi Hideyoshi, who launched an invasion of Kii Province in 1585.

Following the Siege of Osaka in 1615 and the destruction of the Toyotomi clan, Tokugawa Ieyasu donated the buildings of Shoun-ji, a temple which had been built by Hideyoshi to mourn his infant son Tsurumatsu, to Negoro-ji, which had begun to rebuild.

It is believed to have started construction around 1480, and was completed around 1547. based on a record found within the structure during modern dismantling and repairs.