Her speech and personality are powered by an artificial intelligence (AI) system which utilizes a large language model, allowing her to communicate with viewers in the stream's chat.

[6][7] Her responses are generated by a large language model, which are converted into a high-pitched, childlike voice using a text-to-speech application.

[14] On 11 January, the Twitch channel received a temporary ban for unspecified "hateful conduct", likely stemming from controversial statements made by the AI, including skepticism surrounding the validity of the Holocaust during a stream.

[15][16][17] Vedal added more words to her chat filter to prevent Neuro-sama from making offensive statements after this occurred.

[20] On 15 April, Neuro-sama held a stream with Hololive member Takanashi Kiara, during which they rated the contents of viewers' refrigerators.

According to TwitchTracker, the stream reached a peak of 25,687 concurrent viewers, beating the channel's previous record by more than double.

[27] On 4 January, Neuro-sama ended her subathon after 17 days of continuous livestreaming, garnering a total of 41,767 Twitch subscriptions, and 2.43 million collective watch hours.

On 18 March, following a two and a half month break after Neuro-sama's subathon that ended in early January, she returned to streaming to a peak audience of 15,000 viewers with a Shrek watchalong.

During the event, her avatar was displayed on a tablet, and she was carried around the venue by fellow VTubers Camila and Rainhoe to interact live with fans.

[29] On 19 May, Neuro-sama's sister Evil Neuro completed her first full run of Slay the Spire using her adaptive gameplay AI.

The event was hosted on WWE 2K24 in which the VTuber's custom player models compete in a zero-player game style tournament.

[39][40] This comes after Neuro-sama's success in branching out to the Chinese market with her debut on the Bilibili platform back in August 2024;[41] this is in part due to her pre-existing Chinese fans from extensive fansubbing of her Twitch livestreams and due to her unique nature as an AI VTuber; her Bilibili channel grew explosively, even surpassing her Twitch follower count with 700,000+ followers as of January 2025.

[43] On 19 December, Neuro-sama held her second annual subathon, complete with a debut of a new Live2D model, a new original music video named LIFE,[44] her own dedicated merch store page, with a internet-connected lava lamp being a key highlight.

[48] It lasted for a total of 28 days with all subscription goals achieved and amassing over 7 million collective watch hours.

[51] Other aspects of her content delivery include singing songs, playing chess with her viewers, chatting with other VTubers during collaborations and reacting to YouTube videos.

[9][18][52] While playing games or participating in other activities, the AI frequently engages with viewers by responding to their questions and acknowledging donations.